New Seed Extract (SE-PCR) tests for Tomato, Bell pepper, Eggplant, Lettuce and Beet

News publication date: 22 November 2021

We are introducing a new real-time RT-PCR to test the presence or absence of Tomato black ring virus (TBRV) and Beet ringspot virus (BRSV) on seed of Tomato, Paprika, Eggplant, Lettuce and Beet. Furthermore, there was a need to extend the protocol of Tomato yellow leaf curl virus (TYLCV) in addition to Tomato for Paprika and Eggplant. For both these tests is chosen to use the fast, reliable and sensitive Seed-Extract PCR method as test method.

Tomaten aan de plant

To support the sector, Naktuinbouw Laboratories continues to develop new tests that can support diagnosis on plants with symptoms or, on the  other hand, check and guarantee freedom from certain viruses.

The (real-time) PCR is a technique for rapid amplification of DNA

This is done with the help of pathogen-specific building blocks. The increase in DNA can be monitored digitally at any time. This makes it possible to faster and with more certainty confirm whether the seed samples actually contain the pathogen.

Viruses

Tomato black ring virus (TBRV), like Beet ringspot virus (BRSV), is found in Europe, Japan, Saudi Arabia and India. First description of TBRV dates from 1946, later in 1997 BRSV was taxonomically split from TBRV. Both viruses have a very wide host range and are transmitted by the nematode Longidorus. The TBRV test also detects BRSV.

Tomato yellow leaf curl virus (TYLCV), a begomovirus that is transmitted worldwide by whiteflies. The virus is causing major problems in tomatoes. Other crops where the virus also causes problems are peppers, eggplant, potatoes, tobacco and bean.

  • TBRV on seed of tomato, pepper, eggplant, lettuce or beet
    • Sample size: 3,000 untreated seeds
    • Test fee: € 166,00
    • Duration basic test: 4 days
  • TYLCV on seed of tomato, pepper or eggplant
    • Sample size: 3,000 untreated seeds
    • Rate test: € 166,00
    • Duration basic test: 4 days

 

* For basic fees see price list 2021.