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Material for destructive testing

Reviewing a germination test

Shipments of plant samples, seed samples or other risk material for research purposes, which will be destroyed after testing, from outside the European Union (3rd countries) need to be accompanied by a phytosanitary certificate or a letter addressed to the Dutch NPPO (DOIV). 

If a phytosanitary certificate is not available, samples can be imported with a dispensation for receiving samples from third countries, for the purpose of destructive testing. The sample must be accompanied by a letter from the Dutch NPPO (DOIV). You can request this letter from the laboratory by completing the form on this page.

Requirements

  • This option is limited to imports of (plant) material that is not listed as prohibited. An import ban applies to certain varieties of plants, as well as to soil and growing media.  This list states which crops are prohibited.  
  • Material that is already known or detected to be infected with a quarantine organism or an organism worthy of classification as a QO(QO or QwO) may not be imported.
  • The quantity of material submitted must not be larger than necessary for the destructive test.

If the material is already infected with a QO or QwO, you can apply to the NVWA for dispensation from the import prohibition. This is called an Import Licence.

Request DOIV form

You can request the letter by filling in the form below. It is important to fill in the address of the company who will send the samples.

Please note: this is not allowed for Seed Quality analysis. For Seed Quality analysis you need a phytosanitary certificate.

After submitting the request, you will receive the necessary documentation within a few days. You will also receive your shipping label.

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By completing this application, you state that:
* Marked fields are necessary.

Shipment instructions for plant material with a DOIV

    • Your samples must be packed in unbreakable, hermetically sealed leak-proof packaging with maximum of 80kg and a maximum height of 20 cm.
    • Put the attached shipping label on the outside of the package.
    • Put the numbered letter of the Dutch NPPO on the outside of the package, including a warning “Plant Quarantine, do not open”.
    • PLEASE NOTE: do not forget to fill in the packing list information at the back of the form.
    • Put the Laboratory order form together with the sample(s) inside the package.
    • Send the package direct to the address on the attached shipping label. This shipping can only be used for the Naktuinbouw location on Sotaweg 22.
    • Please send not more than the quantity mentioned in your request.
    • Please check whether the courier delivers directly to the Netherlands. If not, please choose another courier. We advise not to use FedEx because there are not sending it directly to the Netherlands.
    • When arranging shipment with your forwarder please use Incoterm DDP - Deliverd Duty Paid to make sure your package is shipped directly to Naktuinbouw without any unnecessary delays and additional transportation costs.
    • Please add a no commercial value (NCV) with the samples.

Frequently asked questions (FAQ)

Where can I find your accreditations?

You can find more information about our accreditations on our website.

Where can I register for newsletters?

You can register for our newsletters on our website.

Which certifications and accreditations does the Naktuinbouw laboratory hold?

The laboratory has three different certifications/accreditations:

  • NEN-EN-ISO 9001:2015
  • NEN-EN-ISO/IEC 17025:2017
  • ISTA Accreditation
Can I also have agricultural crops tested with Naktuinbouw?

For agricultural crops, please contact our fellow inspection service NAK in Emmeloord.

Can I report a resistance after completion of the DUS test?

The answer is yes. Better late than never. We can include your information in our internal plant variety database. This leads to better protection of your plant variety.

Where can I find out how big my seed sample needs to be?

You can find this in the Price list Laboratories 2024 on our website behind the relevant button.

Where can I find out which tests you can perform on my crop?

You will find our current portfolio in the Price list Laboratories 2024. For vegetable crops, which tests we can perform is stated per crop. For plant material, a separate chapter lists which tests are available.

Do you have any questions?
Please contact the Support team of the laboratory

Can I also have tissue propagation material tested for pathogens?

All our tests have been validated on in-vivo material. Depending on the testing technique used, in-vitro material can also be tested. The PCR technique is very sensitive and can generally be used. The ELISA technique is less robust and is not recommended. Preferably have the propagating material tested. Alternatively, again later when the plants have been in the greenhouse for a few weeks.

What type of material do I need for virus testing on plant material?

Virus testing requires young, fully-grown leaves.

Where can I find out which plant material I should have tested for various pathogens?

You can find this in the Price list Laboratories 2024 on our website under the desired test.

Which plant material should be submitted if the plants show symptoms?
  • Take samples from a place where the new symptoms are visible. Running laboratory tests on dead plants is not always possible.
  • Take a sample before any crop protection products are applied. Crop protection products can destroy or mask the symptoms caused by pathogens and interfere with the laboratory tests
  • If the symptoms are unclear, submit the entire plant.
  • If symptoms are unclear or if the plants are uncommon, submit part of a healthy specimen. Pack the healthy sample separately. Indicate that it is healthy material on the label.
  • Prevent leaves and flowers being contaminated by soil or substrate sent with the sample. Place a separate plastic bag around the roots and seal with an elastic band around the stem.
  • Pack the entire sample in paper to prevent dehydration and rotting. Paper will regulate the moisture content in the plastic bag.
How do I know how many plants I should sample for export?

For export, an inspector often arranges the sampling. You can send an email to planning@naktuinbouw.nl. They can tell you exactly what you need to do.

What is the difference between disinfected and treated seeds?
  • Disinfected seeds carry residues to a greater or lesser extent. The most obvious example is seeds coated with crop protection agents. The aim is to remove as much fungi and / or bacteria from the seeds as possible. These products may potentially influence the tests.
  • Treated (disinfected) seeds do not contain any residues. Examples include warm water treatment or treatment with TSP or HCl. These products are not expected to affect the tests.
Can I also have disinfected/coated seeds tested?

The tests used for seed health have been developed and validated for untreated seeds. Seed treatments can affect the reliability of the health test results. For this reason, preferably submit untreated seed samples. If there is an express wish/necessity to test treated seeds, a comment on the certificate will state that the test has only been validated on untreated seeds.

This is not applicable to tests that explicitly state that the test is performed on treated seed. Please state the type of seed treatment applied when submitting the sample.

This plays a less important role with quality tests (germination power, purity etc.).

Can I also have primed seeds tested?

The tests used for seed health have been developed and validated for untreated seeds. Seed treatments can affect the reliability of the health test results. For this reason, preferably submit untreated seed samples. This especially applies to tests performed using a culture method. The number of microorganisms in the seed increases sharply during the priming process. These additional microorganisms in the seed disrupt the test to such an extent that, even after additional disinfection after priming, no assessable results can be obtained.

This plays a less important role with quality tests (germination power, purity etc.).

Can Naktuinbouw disinfect the seeds before a health test?

Unfortunately there are two reasons that prevent this:

  • Naktuinbouw has no facilities for the application of disinfectants, other than those necessary to perform the test.
  • If a sample is disinfected, it is no longer the actual sample that was submitted. As such, it is no longer representative of the lot from which the sample was taken.
Can samples disinfected with Thiram be sent in for laboratory testing?

We are only allowed to receive and test the samples if seed lots have been treated with Thiram and are going to countries outside the EU.

Can samples be returned?

Samples intended for health tests cannot be returned. First of all, these samples are tested destructively. After testing, the samples are no longer usable. Secondly, they are in a contained area. The samples are not allowed to leave this area.

Which tests does Naktuinbouw perform?

You will find our current portfolio in the Price list Laboratories 2024. For vegetable crops, which tests we can perform is stated per crop. For plant material, a separate chapter lists which tests are available.

Can Naktuinbouw test for CLVd, PCFVd, PSTVd, TASVd, TCDVd and TPMVd?

When Pospiviroids test are requested, the seeds are tested for the following viroids:

  • Columnea latent viroid (CLVd)
  • Pepper chat fruit viroid (PCFVd)
  • Potato spindle tuber viroid (PSTVd)
  • Tomato apical stunt viroid (TASVd)
  • Tomato chlorotic dwarf viroid (TCDVd)
  • Tomato planta macho viroid (TPMVd)

If plant material is submitted, in addition to the viroids above-mentioned, tests are also performed for:

  • Chrysanthemum stunt viroid (CSVd)
  • Iresine viroid 1 (IrVd)
  • Portulaca latent viroid (PoLVd)
Can Naktuinbouw test for phytoplasmas?

A generic PCR test is available for testing for phytoplasmas. This can detect the following phytoplasmas:

  • Apple proliferation phytoplasma; syn. Candidatus Phytoplasma mali
  • Apricot chlorotic leafroll mycoplasma
  • Aster yellows
  • Elm phloem necrosis
  • Elm yellows
  • European stone fruit yellows; syn. Candidatus Phytoplasma prunorum
  • Peach rosette phytoplasma
  • Peach X disease phytoplasma
  • Peach yellows phytoplasma
  • Pear decline phytoplasma
  • Pear decline phytoplasma; syn. Candidatus Phytoplasma pyri
  • Potato stolbur
  • Rubus stunt
  • Strawberry green petal phytoplasma
  • Strawberry witches’ broom
  • Yellow decline of Lavendula
The country requirements for Australia state that specific PCR methods must be used to test for Xylella.

The Harper and Quyang method are both used to test for Xylella. Both are required by Australia. This means that our protocol complies with Australian requirements.

Can Naktuinbouw identify which disease my plants have?

Samples with symptoms are tested by the Diagnostics department. Various techniques are used to detect and identify which pathogen is present. If you already suspect which pathogen(s) may be present, a specific test for those pathogens is also possible.

Can my crop be made virus-free?

It is possible to make certain crops virus-free (strawberry, small fruit and woody fruit crops). See the price list Laboratoria 2024 for more details. For all other crops, please contact specialised tissue culture companies.

Which certifications and accreditations does the Naktuinbouw laboratory hold?

The laboratory has three different certifications/accreditations:

  • NEN-EN-ISO 9001:2015
  • NEN-EN-ISO/IEC 17025:2017
  • ISTA Accreditation
Where can I find your accreditations?

You can find more information about our accreditations on our website.

Can I also have agricultural crops tested with Naktuinbouw?

For agricultural crops, please contact our fellow inspection service NAK in Emmeloord

My results state that my bean sample is overgrown with saprophytes. What can I do?

Leave the lot for a few months, then send a sample again. Saprophytes cannot survive on bean seeds for long.

My results state that my sample is overgrown with saprophytic bacteria or fungi. What does this mean?

Saprophytic bacteria or fungi are harmless organisms that sometimes naturally occur on seeds. If these saprophytes are present in high numbers, this may disturb the culture method. This means there is a considerable risk that an assessment of that test will no longer be possible.

My results state that my tomato sample is overgrown with saprophytes. How is that possible?

Drying after HCl treatment must be done quickly. If the drying process takes too long, the risk of saprophytic bacteria and fungi growing is too high.

Pectinase treatment is also an option. This is faster and reduces the exposure time and the growth of saprophytes.

What does the result 'Follow-up test' on my certificate mean?

The test where this is mentioned is the basic test. Suspicious values were found in this test and a (an additional) validation test was performed. You can find this on the certificate with the same sample, but it states a different technique. The result of this is leading.

There is an incorrect information on my certificate. Can that be adjusted?

Certain amendments can be made after a certificate has been issued. Please send a request to Laboratory Support team: laboratoria@naktuinbouw.nl.

According to our ISO certification, we are obliged to change the version number on the new certificate. It also states which amendments have been made.

Is the minimum germination rate subject to any directives or legislation?

No specific directives or legislation apply. The specific requirements are set by companies themselves.

When can I expect the results of my test(s)?

The Price list Laboratories 2024 states the duration of the tests. Tests generally start within three weeks of receiving the samples. However, this time may differ if a high number of samples are submitted. MyNaktuinbouw shows the expected end date and any results of your completed test(s).

Can Naktuinbouw issue a NAL certificate?

NAL stands for Naktuinbouw Authorized Laboratory. This means that Naktuinbouw authorises companies to test their own lots of material. The certificates issued by these companies are accepted in the same way as a Naktuinbouw certificate for the purpose of obtaining a phytosanitary certificate.

Naktuinbouw does not issue NAL certificates.

Where should I send my samples?

Samples can be sent by PostNL to:

Testing Laboratory
Attn. Laboratories Administration
P.O. Box 40
2370 AA Roelofarendsveen, NL

Diagnostics
Naktuinbouw
Attn: Diagnostics
Sotaweg 22
2371 AA Roelofarendsveen, NL

Variety Tracer
Naktuinbouw
Attn: Variety Tracer
P.O. Box 40
2370 AA Roelofarendsveen

When sending the samples with a courier service, or in case of large quantities of samples (e.g. on a pallet), please use the visiting address:

Testing Laboratory
Naktuinbouw
Attn: Laboratory Administration
Sotaweg 22
2371 GD Roelofarendsveen

Diagnostics
Naktuinbouw
Attn: Diagnostics
Sotaweg 22
2371 GD Roelofarendsveen

Variety Tracer
Naktuinbouw
Attn: Variety Tracer
Sotaweg 22
2371 GD Roelofarendsveen

Samples can be received between 8:00-17:30, from Mondays to Fridays.

Bioassays
Samples may be sent to:

Naktuinbouw
Tienrayseweg 9a
5961 NK Horst

Which documents should I to send with my samples?

A submission form downloaded from the website. We have three different submission forms:

  1. For plant material with symptoms
  2. For plant material without symptoms
  3. For Seed samples

Samples submitted from outside the EU must be accompanied by a phytosanitary certificate or DOIV.

Where can I find a submission form?

You can find this form on the website. There are three different types:

Is a phytosanitary certificate required?

A phytosanitary certificate is sometimes required for samples submitted from outside the EU. If it is not possible to request a phytosanitary certificate, you can request a special, accompanying letter (DOIV) on the website.

When can I send my samples?

We advise you to send plant samples (perishable material) without symptoms on Mondays or Tuesdays. Plant samples with symptoms can be sent from Mondays to Thursdays. This will avoid samples being left in the post over the weekends and not being stored in the correct conditions. Samples sent on these days will also arrive in time for testing.

Seed samples can be sent every day of the week.

I am submitting samples to Naktuinbouw for the first time. Which documents should I include?

A registration form must be filled in first so we can enter the necessary company details in our system. 

Can more than one crop be listed on a DOIV?

If the different crops are in the same package, one DOIV is sufficient. A separate DOIV must be requested for each shipment.

When do I have to request a DOIV?

A DOIV must be requested if it is not possible to request a phytosanitary certificate. The application must comply with the following criteria:

  • The crop/family is not stated on the EU list of banned plants, unless indicated that the material is intended for planting up.
  • The material is used for destructive testing.
  • The material is sent directly to the Netherlands from the country of origin, without transhipment in another country.
What is a DOIV?

A DOIV is a document issued by the Netherlands Food and Consumer Product Safety Authority (NVWA). This document permits plant material without a phytosanitary certificate to be imported for the purpose of destructive testing on samples. The Dutch customs authorities required this document to release the shipment.

Where should I send my samples?

Samples can be sent by PostNL to:

Testing Laboratory
Attn. Laboratories Administration
P.O. Box 40
2370 AA Roelofarendsveen, NL

Diagnostics
Naktuinbouw
Attn: Diagnostics
Sotaweg 22
2371 AA Roelofarendsveen, NL

Variety Tracer
Naktuinbouw
Attn: Variety Tracer
P.O. Box 40
2370 AA Roelofarendsveen

When sending the samples with a courier service, or in case of large quantities of samples (e.g. on a pallet), please use the visiting address:

Testing Laboratory
Naktuinbouw
Attn: Laboratory Administration
Sotaweg 22
2371 GD Roelofarendsveen

Diagnostics
Naktuinbouw
Attn: Diagnostics
Sotaweg 22
2371 GD Roelofarendsveen

Variety Tracer
Naktuinbouw
Attn: Variety Tracer
Sotaweg 22
2371 GD Roelofarendsveen

Samples can be received between 8:00-17:30, from Mondays to Fridays.

Bioassays
Samples may be sent to:

Naktuinbouw
Tienrayseweg 9a
5961 NK Horst

Instructions for taking samples
  • Take samples from a spot where the new symptoms are visible. It is often not possible to run laboratory tests on plants that have died off completely.
  • Take a sample before any pesticides/crop protection agents are applied. Pesticides can destroy or mask the symptoms caused by pathogens and interfere with the laboratory tests.
  • If the symptoms are unclear, send us the entire plant.
  • Where symptoms are unclear, or in the case of a rare plant, please also send us part of a healthy specimen. Pack the healthy sample separately and indicate on the label that it is healthy material.
  • If it is impossible to send a sample immediately, keep it cool. Never place samples in direct sunlight.
Which documents should I to send with my samples?

A submission form downloaded from the website. Samples submitted from outside the EU must be accompanied by a phytosanitary certificate or DOIV.

Where can I find a submission form?

You can find this form on the website. There are three different types:

Is a phytosanitary certificate required?

A phytosanitary certificate is sometimes required for samples submitted from outside the EU. If it is not possible to request a phytosanitary certificate, you can request a special, accompanying letter (DOIV) on the website.

When can I send my samples?

We advise you to send plant samples (perishable material) without symptoms on Mondays or Tuesdays. Plant samples with symptoms can be sent from Mondays to Thursdays. This will avoid samples being left in the post over the weekends and not being stored in the correct conditions. Samples sent on these days will also arrive in time for testing.

Seed samples can be sent every day of the week.

I am submitting samples to Naktuinbouw for the first time. Which documents should I include?

A registration form must be filled in first so we can enter the necessary company details in our system. 

It is also important to send an order form with the samples. This form is also on our website.

I cannot find my test in the price list. Can you still perform it?

The Price list Laboratories 2024 states our standard portfolio of tests. For export purposes, some countries require tests for which no validated protocol is available. In exceptional cases, based on our experience and knowledge of crops and pathogens, we can create a customised test. If the test proceeds as expected, the result can be used for export purposes.

To request a test, please contact the Front Office of the laboratory.

Can my crop be made virus-free?

It is possible to make certain crops virus-free (strawberry, small fruit and woody fruit crops). See the Price list Laboratoria 2024 for more details. For all other crops, please contact specialised tissue culture companies.

Is the minimum germination rate subject to any directives or legislation?

No specific directives or legislation apply. The specific requirements are set by companies themselves.

Fee

Here you can find the current list of fees of Naktuinbouw Laboratory:

Price list Laboratories 2024

How do I apply for a DOIV?

You ask the laboratory for this letter by completing this form. When importing plant or seed samples for destructive testing from outside the EU (third countries), a phytosanitary certificate or an accompanying letter from the NVWA is required.

How do I register as a Naktuinbouw customer?

To register as a Naktuinbouw customer to use our Variety Testing services, please complete this form.