In order to achieve international harmonisation of the testing protocol for the detection of pospiviroids in tomato and sweet pepper seeds, Naktuinbouw makes its protocols available to other parties. We also have a compliance available about the testing method used for Clavibacter testing.
These protocols can be used for detecting CEVd, CLVd, MPVd, PCFVd, PSTVd, TASVd, TCDVd and TPMVd. The protocols were developed in a 3x1000 seed format and are also available in a 50x400 seed format to meet Australian requirements. They involve manual RNA extraction (for sweet pepper seeds) or KingFisher extraction (for tomato seeds). The RT-PCR is based on a combination of a number of multiplex PCRs for detecting eight pospiviroids. The detection of PSTVd-TCDVd is based on the current Naktuinbouw protocol for these viroids, which has been validated. The entire protocol will be validated in the EU-TESTA project.
The laboratory has three different certifications/accreditations:
For agricultural crops, please contact our fellow inspection service NAK in Emmeloord
Leave the lot for a few months, then send a sample again. Saprophytes cannot survive on bean seeds for long.
Saprophytic bacteria or fungi are harmless organisms that sometimes naturally occur on seeds. If these saprophytes are present in high numbers, this may disturb the culture method. This means there is a considerable risk that an assessment of that test will no longer be possible.
Drying after HCl treatment must be done quickly. If the drying process takes too long, the risk of saprophytic bacteria and fungi growing is too high.
Pectinase treatment is also an option. This is faster and reduces the exposure time and the growth of saprophytes.
The test where this is mentioned is the basic test. Suspicious values were found in this test and a (an additional) validation test was performed. You can find this on the certificate with the same sample, but it states a different technique. The result of this is leading.
Certain amendments can be made after a certificate has been issued. Please send a request to Laboratory Support team: firstname.lastname@example.org.
According to our ISO certification, we are obliged to change the version number on the new certificate. It also states which amendments have been made.
No specific directives or legislation apply. The specific requirements are set by companies themselves.
NAL stands for Naktuinbouw Accredited Laboratory. This means that Naktuinbouw authorises companies to test their own lots of material. The certificates issued by these companies are accepted in the same way as a Naktuinbouw certificate for the purpose of obtaining a phytosanitary certificate.
Naktuinbouw does not issue NAL certificates.