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New real-time RT-PCR tests for plant material for raspberry and blackberry

Publicatiedatum: 31 januari 2024

We introduce the new real-time RT-PCR for the detection of a set of viruses occurring in raspberry and blackberry.

The real-time RT-PCR is a technique for rapid amplification of RNA

This is done with the help of pathogen-specific building blocks. The amplification in RNA can be monitored digitally at any time. This makes it possible to confirm quicker and with more certainty whether the pathogen is present.

Viruses

Below five viruses are described, which all can occur in Rubus spp. like raspberry and blackberry:

  1. Black raspberry necrosis virus (BRNV) occurs worldwide and is transmitted by specific aphids like the European large raspberry aphid (Amphorophora idaei) in Europe and American large raspberry aphid (Amphorophora agathonica) in Amerika.
  2. Raspberry bushy dwarf virus (RBDV) also occurs worldwide. The spread within a field occurs with infected pollen. The virus is seed transmitted.
  3. Raspberry leaf mottle virus (RLMV) occurs again worldwide en is – just like BRNV – spread by specific Rubus spp. Aphids. Research indicated that Raspberry leaf spot virus and Raspberry mottle virus are strains of RLMV.
  4. Rubus yellow net virus (RYNV) occurs worldwide and is transmitted by aphids; in this case European and American large raspberry aphid.
  5. Raspberry vein chlorosis virus (RVCV), occurs mainly in Europe, but it is less common than above mentioned viruses. It is spread by aphids, in this case the raspberry aphid (Aphis idaei).

Above mentioned viruses can alle spread with vegetatively propagated plant material.

New tests for plant material Horticultural crops

  • BNRV, RBDV, RLMV, RYNV and RVCV
    • Sample: Max. 25 leaves
    • Test fee: € 109
    • Duration test: 4 days

Naktuinbouw Laboratories supports sector

We continue to support the sector by developing new tests to help diagnose plants with symptoms or to check for, and guarantee, absence of certain viruses. *For basic fees, see the 2024 price list [link]

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