New real-time RT-PCR assays for testing plant material of horticultural crops

News publication date: 22 November 2021

To support the sector, Naktuinbouw Laboratories continues to develop new tests that can support diagnosis of plants with symptoms or, on the contrary, check and guarantee absence of certain viruses. We are introducing a new TaqMan RT-PCR assays for detection of TRSV and ToRSV in plant material of horticultural crops.


The real-time RT-PCR is a technique for rapid amplification of RNA

For this, pathogen-specific building blocks are used. The increase in RNA can be digitally monitored through time. The assays are faster and give more certainty on presence (or absence) of the viruses.


Tobacco rinspot virus (TRSV) and tomato ringspot virus (ToRSV) are transmitted by the nematode Xiphinema americanum. The nematode as well as the viruses are on the EU quarantine list. The nematode does not occur in Europe, but the viruses can travel with plant material and are occasionally detected. TRSV and ToRSV have a wide host-plant range in vegetable, ornamental and nursery stock. The protocols for these quarantine organisms are approved by the Dutch NPPO.

New assays for testing plant materiaal horticultural crops

  • TRSV or ToRSV in plant material
    • Sample: Max. 25 leaves
    • Rate PCR assay: € 71,50
    • Time span test: 4 days


* For basic fees see Price list Laboratories 2022.