New seed extract PCR (SE-PCR) test for various legume crops

Publicationdate: 10 december 2025

Starting December 1st, we can test for the presence or absence of Curtobacterium flaccumfaciens pv. flaccumfaciens on seeds of bean (Phaseolus vulgaris) seeds, runner bean (Phaseolus coccineus), Lima bean (Phaseolus lunatus), Adzuki bean (Vigna angularis), Urad bean (Vigna mungo), Mung bean (Vigna radiata), pea (Pisum sativum), soybean (Glycine max) and broad bean (Vicia faba). For this test is chosen to use the fast, reliable and sensitive seed-extract PCR method as test method. The existing cultivation method on beans has also been expanded to include the above-mentioned crops.

Technique for rapid amplification of DNA

This is done with the help of pathogen-specific building blocks. The increase in DNA can be monitored digitally at any time. This makes it possible to faster and with more certainty confirm whether the seed samples actually contain the pathogen.

Curtobacterium flaccumfaciens pv. flaccumfaciens

This bacterium is the causative agent of bacterial wilt in species of the legume family, including the common bean. It was first observed in 1922 in South Dakota, USA, after which it spread to other US states, South America, Iran, South-east Asia, Africa, Europe, and Australia. Initially, only a yellow variant was reported, but over the years, orange, red, and even purple variants of the bacterium have also been found. This seed-borne pathogen can cause field production losses of 20-50%.

The test

Curtobacterium flaccumfaciens pv. flaccumfaciens on seeds of various legume crops

Test code: CFFQ050

Sample: 5.000 untreated seeds

Rate test: € 279,50

Duration test: 4 days

For basic fees see price list.

Naktuinbouw Laboratories supports sector

To support the sector, Naktuinbouw Laboratories continues to develop new tests that can support diagnoses on plants and seeds with symptoms or just checking for and guaranteeing absence from certain plant pathogens.

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