New seed extract PCR tests for Cucurbitacea, pepper and New Zealand spinach seeds

Publicationdate: 22 september 2025

Starting September 1st, we can test for the presence or absence of Papaya ringspot virus (PRSV) on seed of Cucurbitaceae and Cucumber mosaic virus (CMV) on pepper and New Zealand spinach seeds. For this test is chosen to use the fast, reliable and sensitive seed-extract PCR method as test method.

The (real-time) PCR is a technique for rapid amplification of DNA

This is done with the help of pathogen-specific building blocks. The increase in DNA can be monitored digitally at any time. This makes it possible to faster and with more certainty confirm whether the seed samples actually contain the pathogen.

About the viruses

Cucumber mosaic virus (CMV)

CMV is a Cucumovirus that can infect over 850 plant species worldwide, including pepper and New Zealand spinach. The virus is mainly spread by aphids, but can also be transmitted via seeds and mechanical contact. In Europe, CMV is classified as a Regulated Non-Quarantine Pest (RNQP), which means seeds must be tested for this virus before use.

Papaya ringspot virus (PRSV)

PRSV is a Potyvirus that causes significant damage to plant production worldwide. It primarily infects papaya and plants from the Cucurbitaceae family. The virus is spread by aphids. In countries such as Egypt, Bahrain, and Jordan, PRSV is listed on the EPPO A2 list, meaning it is considered a quarantine pathogen. Therefore, seed testing is mandatory for export to these countries.

Our tests

CMV on seed of pepper and New Zealand spinach

Test code: CMVQ030

Sample size: 3,000 untreated seeds

Test fee: € 259,00

Duration test: 4 days

PRSV on seed of Cucurbitaceae

Test code: PRSVQ030

Sample size: 3,000 untreated seeds

Test fee: € 259,00

Duration test: 4 days

For basic fees see price list.

Naktuinbouw Laboratories supports sector

To support the sector, Naktuinbouw Laboratories continues to develop new tests that can support diagnoses on plants and seeds with symptoms or just checking for and guaranteeing absence from certain plant pathogens.

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