New Seed Extract PCR (SE-PCR) test for lettuce

Publicationdate: 25 juni 2025

We are introducing a new real-time PCR to test the presence or absence of Lettuce mosaic virus (LMV) on seeds of lettuce. This test will replace the seed extract ELISA test as of July 1, 2025.

This test gives no indication of whether or not the virus is infectious. In case of a positive result, a new sample can be sent in to perform a seedling ELISA test.

A technique for rapid amplification of RNA

This is done with the help of pathogen-specific building blocks. The increase in DNA can be monitored digitally at any time. This makes it possible to faster and with more certainty confirm whether the seed samples actually contain the pathogen.

Lettuce mosaic vius (LMV)

The Lettuce mosaic virus results in yellowing and necrosis of the leaves, growth distortions and dwarfisms of lettuce plants. The pathogen is globally distributed, it often transmitted through aphids and can be seed transmitted. Some LMV strains can also overcome resistance in lettuce plants, being of greater concern.

LMV on seed of lettuce

Sample size: 3,000 untreated seeds

Test fee: € 259

Duration test: 4 days

For basic fees see price list 2025.

We support the sector

To support the sector, Naktuinbouw Laboratories continues to develop new tests that can support diagnosis on plants with symptoms or, on the other hand, check and guarantee freedom from certain plant pathogens.

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