Bacterial test on cabbage seed has been optimized

News publication date: 18 May 2020

The test for detecting Xanthomonas campestris pv. Campestris (Xcc) and Xanthomonas campestris pv. raphani (Xcr) on cabbage seed (Brassica oleracea L.) has been further optimized. We did this by adding a spike extraction step to the dilution plating method. With this optimization we follow the international standard.

Kool

As of May 1, 2020 we perform the Xcc/Xcr test with spike only

This applies for both treated (warm water treatment) and untreated seeds. All samples registered at our laboratory after 1 May will be tested with the new method.

The dilution plating test may be confirmed by follow-up tests

If positive colonies are found in the dilution plating test we perform a real-time PCR as usual with this test. If the PCR does not give a clear result, a bio assay will follow.

You can submit different sample sizes at our laboratory

We test samples in multiples of (minimum) 10,000 seeds. By default, we test samples of 10,000, 30,000, 50,000 or 100,000 seeds. You can submit untreated or treated seeds. We kindly ask you to indicate on the submission form whether the seeds have been treated.

The test fees have been adjusted

With the added spiking the test is more laborious than the previous method. That is why we have increased the fees for the new PCR tests. Below you find the new test rates (excluding basic fees):

Untreated seeds:

  • 10,000 seeds: € 189.10
  • 30,000 seeds: € 226.90
  • 50,000 seeds: € 331.20
  • 100,000 seeds: € 460.70

Treated seeds:

  • 10,000 seeds: € 234.50
  • 30,000 seeds: € 268.30
  • 50,000 seeds: € 390.00
  • 100,000 seeds: € 527.90