Nematodes

Naktuinbouw can test the quality of soil, seed and plant material for the presence of nematodes. These tests give you certainty as to the quality of your propagating material. Nematodes can cause extensive damage in crops. Preventive testing of soil, seed and plant material can prevent this. In addition, we can perform a nematode test on your material for a nematode-free declaration for certain export destinations, for example.

About the test

Naktuinbouw can test for various plant parasitic nematodes, including Xiphinema and Longidorus species in soil, for the Naktuinbouw Elite quality-plus system in strawberries, for example.

In order to trade onion sets (seed), a nematode-free declaration for stem nematodes (Ditylenchus dipsaci) is required. Other crops are also required to be accompanied by a nematode-free declaration for certain export destinations.

We use an Oostenbrink elutriator to flush out and analyse all live nematodes. This is a requirement of the Select Plant quality-plus system for strawberries, for example. For this purpose, our lab technicians test soil used for strawberries for the presence of Xiphinema spp. and Longidorus spp.

They can also reveal nematodes, including stem nematodes (Ditylenchus dipsaci), in onion sets (seed). A misting device is used to extract the nematodes from plant material and seed. This method uses the motility and sedimentation speed of the nematodes. When infected plant material or seed is placed in water, the nematodes crawl out of the material in the water and sink to the bottom. The lab technician can then identify them.

Naktuinbouw can identify the following nematodes:

  • Aphelenchoides spp.
  • Aphelenchus spp.
  • Ditylenchus spp. (stem nematode)
  • Hemicycliophora spp.
  • Heterodera larvae
  • Longidorus spp.
  • Meloidogyne spp. (root-knot nematode)
  • Paratylenchus spp.
  • Pratylenchus spp. (root lesion nematode)
  • Roylenchus spp.
  • Trichodorus spp. (virus vector nematode)
  • Xiphinema spp.

If you don't yet know which test you want to have performed, check out here what kinds of tests Naktuinbouw can perform for you.

Requirements for submission

General submission requirements for samples:

  • Pack the material in plastic.
  • Send the material in a sturdy box. Attach the submission form to the outside of the box.
  • For moisture content determination in seed samples, please pack and send these in moisture-proof packaging.
  • If you mark your submission URGENT, the sample will be prioritised and dealt with as soon as possible. We will then charge the urgency fee (see list of fees).

In addition to the general submission requirements, there are also requirements relating to the material you submit.

Instructions for submitting seeds

  • For Naktuinbouw certificates, always send in at least twice the necessary amount of seed. This will ensure that there are no delays if a test has to be repeated.
  • If possible, always submit untreated seed samples. Seed treatment can affect the reliability of the health test results.
  • State on the submission form whether the seed has been disinfected.

Instructions for submitting leaf or stem material

  • Ideally we would like to receive your material by no later than the Wednesday before the week of the test.
  • Please do not send material on a Friday as it cannot be processed over the weekend.
  • For potyvirus testing, we require double the amount of material.

Shipping and delivery address for samples for testing:

Naktuinbouw

Laboratory Administration

Sotaweg 22

2371 GD Roelofarendsveen, the Netherlands

Fee

In our list of fees you will find details of the fees for the various tests we can perform for you in a range of areas.

Application

You can use the submission form to submit your sample for analysis. Send it in with your samples.

Questions?