NEW: TaqMan RT-PCR for tobaccos rattle virus in horticultural crops

News publication date: 13 November 2019

The test laboratory has recently added a new PCR test to its portfolio for tobacco rattle virus (TRV). The symptoms can vary per crop. On most crops, chlorotic mottling and line patterns occur. The test has been validated for plant material of horticultural crops.

Tobacco rattle virus is spread by stubby-root nematodes (Trichodoridae) which makes the PCR very suitable for, among other things, perennial crops grown in full soil. The virus may occur in another form that is not detected by the ELISA method.

Testing for this virus using a PCR method is mandatory within the Naktuinbouw Elite quality-plus system for perennials, due to the risk of infection. The PCR is more sensitive than the existing ELISA method and more leaves can be combined per sample. In addition, similar to the ELISA method, this PCR test also detects all variants of the virus.

  • Sample: 25 pieces of leaf (max. 1 gram in total) can be collected in one extraction bag (brand Bioreba), or 25 whole leaves in a sealed plastic bag.
  • Fee PCR test: €64.80. The sliding scale fees for the PCR test are different from the ELISA. For the list of basic fees, see the price list.
  • Duration of the test: 3 days

The ELISA method can also be requested. This test is cheaper, and the duration of the test is the same. The TRV ELISA is often combined with the pea early browning virus (PEBV). This virus is related to TRV and the PEBV ELISA also detects variants of TRV. Both ELISA tests are necessary to detect the different variants of TRV, the PCR detects all variants of TRV.

The PCR test can be requested in combination with the cucumber mosaic virus (CMV) test on the same sample.

See the price list Laboratories for basic fees.