Current PCR method replaced by real-time PCR

News publication date: 18 September 2019

For a number of pathogen/crop combinations, the conventional colony verification PCR has been replaced by the real-time quantitative PCR method (Taqman qPCR).

Huidige PCR-methode

Real-time PCR is a technique that multiplies the DNA. Using this technique, the increase in DNA can be monitored digitally at any moment. This is done with the help of pathogen-specific building blocks. This enables faster and more accurate verification of whether suspect colonies actually concern the pathogen. Fewer hazardous substances are also required to obtain a result, making the technique not only faster, but also safer for people and the environment.

The PCR method has been replaced for the following 3 tests

  1. Pseudomonas syringae pv. maculicola on cabbage seeds (Brassica oleracea L.) and radish (Raphanus sativus L.)
    • Sample: 2,500/7,500/10,000 untreated seeds or 10,000/30,000 treated seeds
    • Price PCR follow-up test: € 60.70 (unchanged)
    • Duration of the test: 15 days
  2. Pseudomonas syringae pv. tomato on tomato seeds (solanum lycopersicum
    • Sample: 10,000/30,000 untreated seeds
    • Price PCR follow-up test: € 60.70 (unchanged)
    • Duration of the test: 16 days
  3. Xanthomonas hortorum pv. carotae on carrot seeds (Daucus carota)
    • Sample: 10,000/20,000 untreated seeds
    • Price follow-up test: € 60.70 (unchanged)
    • Duration of the test: 16 days

See the pricelist for basic fees.